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KMID : 0043319960190060514
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Archives of Pharmacal Research
1996 Volume.19 No. 6 p.514 ~ p.519
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Effects of Hydroxylated Flavonoids on the Ethoxyresorufin O-deethylase and Benzo()pyrene Hydroxylase
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Han Sun-Ho
Sheen Yhun-Yhong
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Abstract
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In order to understand the mechanism of action of flavonoids on the drug metabolizing enzyme, cytochrome P450IA1, this study was undertaken to examine the effect of chrysin, morin, myricetin and aminopyrine on the activities of ethoxyresorufin O-deethylase and benzo(.alpha.) pyrene hydroxylase in the liver. In the isolated perfused rat liver that was pretreated with 3-methylcholanthrene (3MC), chrysin, morin, myricetin and aminopyrine inhibited the activity of ethoxyresorufin O-deethylase with concentration dependent manner. The isolated liver perfusion with chrysin, morin, myricetin and aminopyrine showed inhibition on the induction of ethoxyresorufin O- deethylase by 3MC. And also, in mouse liver hepa I cells, 3MC-stimulated the benzo(.alpha.)pyrene hydroxylase activity which was inhibited by chrysin, morin, myricetin and aminopyrine. These results strongly suggested that hydoxylated flavonoids interfered not only the induction of cytochrome P45OIA1 enzymes by 3MC but also the interaction of substrates and enzyme.
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KEYWORD
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Ethoxyresorufin O-deethylase, Benzo(a)pyrene hydroxylase, 3-methylcholanthrene, Cytochrome P450, Flavonoic
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